An Increased Diagnostic Sensitivity of Truncated GAD65 Autoantibodies in Type 1 Diabetes May Be Related to HLA-DQ8

• DOI: 10.2337/db16-0891
• Publication Date: 2016-12-28T01:40:53Z
• Authors: Axel Wester; Hanna Skärstrand; Alexander Lind; Anita Ramelius; Annelie Carlsson; Elisabeth Cedervall; Björn Jönsson; Sten A. Ivarsson; Helena Elding Lar...; Karin Larsson; Bengt Lindberg; Jan Neiderud; Malin Fex; Carina Törn; Åke Lernmark
• Keywords: Male; Adolescent; Glutamate Decarboxylase; Infant; Sensitivity and Specificity; Peptide Fragments; 3. Good health; Young Adult; 03 medical and health sciences; Diabetes Mellitus, Type 1; 0302 clinical medicine; Haplotypes; Case-Control Studies; Child, Preschool; HLA-DQ Antigens; Humans; Female; Child; Autoantibodies

N-terminally truncated (96–585) GAD65 (tGAD65) autoantibodies may better delineate type 1 diabetes than full-length (fGAD65) autoantibodies. We aimed to compare the diagnostic sensitivity and specificity between fGAD65 tGAD65 for in relation HLA-DQ. Sera from children adolescents with newly diagnosed (n = 654) healthy control subjects 605) were analyzed radiobinding assays (fGADA), (tGADA), commercial 125I-GAD65 (RSRGADA) The receiver operating characteristic curve did not differ fGADA tGADA. At optimal cutoff, was lower tGADA at similar specificities. In 619 patients, 64% positive RSRGADA compared 68% 74% Using non-DQ2/non-DQ8 patients as reference, risk of being increased DQ2/2 DQ2/8. Notably, logistic regression analysis suggested that DQ8/8 had an be (P 0.003) 0.09). a higher both RSRGADA. As represent 10–11% <18 years age, should prove useful disease classification.