Adiponectin Increases Glucose-Dependent Electrical Activity and Insulin Secretion in Mouse Pancreatic ß Cells

• DOI: 10.2337/db18-329-or
• Publication Date: 2018-06-22T18:57:33Z
• Authors: JOANA I. REAL; BELEN CHANCLON GA...; INGRID WERNSTEDT ...; PATRIK RORSMAN
• Keywords: Pancreatic Islets

Decreased plasma adiponectin has been implicated as a cause of diabetes. However, the functional impact on glucose-dependent responses β-cells remains poorly understood. Here we have investigated whether chronically and acutely increased levels affect electrical activity (sharp electrodes) insulin secretion in using respectively, overexpressing (APNtg) mice acute application adiponectin. In exposure experiments, (20 µg/ml, 11 mM glucose) 42% β-cell (measured fraction ‘active phase’) 32%. islets from APNtg 44% active phase 25%. The effects correlated with membrane hyperpolarization resting potential at 0 glucose averaged -60±2 control -69±2 β-cells. There was moderate effect action width (<20%) more pronounced frequency by exogenous (+35%) (+50%).These results suggest that both positive pancreatic Disclosure J.I. Real: None. B. Chanclon Garcia: I. Wernstedt Asterholm: P. Rorsman: