RNF20 Functions as a Transcriptional Coactivator for PPARγ by Promoting NCoR1 Degradation in Adipocytes

• DOI: 10.2337/db19-0508
• Publication Date: 2019-10-11T22:25:11Z
• Authors: Yong Geun Jeon; Jae Ho Lee; Yul Ji; Jee Hyung Sohn; Dabin Lee; Dong Wook Kim; Seul Gi Yoon; Kyung Cheul Shin; Jeu Park; Je Kyung Seong; Je-Yoel Cho; Sung Sik Choe; Jae Bum Kim
• Keywords: Corepressor

Adipose tissue is the key organ coordinating whole-body energy homeostasis. Although it has been reported that ring finger protein 20 (RNF20) regulates lipid metabolism in liver and kidney, roles of RNF20 adipose have not explored. Here, we demonstrate promotes adipogenesis by potentiating transcriptional activity peroxisome proliferator–activated receptor-γ (PPARγ). Under normal chow diet feeding, Rnf20 defective (Rnf20+/−) mice exhibited reduced fat mass with smaller adipocytes compared wild-type littermates. In addition, high-fat diet–fed Rnf20+/− alleviated systemic insulin resistance accompanied a expansion tissue. Quantitative proteomic analyses revealed significantly decreased levels PPARγ target proteins mice. Mechanistically, promoted proteasomal degradation nuclear corepressor 1 (NCoR1), which led to stimulation PPARγ. Collectively, these data suggest RNF20-NCoR1 novel axis adipocyte biology through fine-tuning