Temporal Analysis of Amylase Expression in Control, Autoantibody-Positive, and Type 1 Diabetes Pancreatic Tissues

• DOI: 10.2337/db19-0554
• Publication Date: 2019-10-09T22:05:13Z
• Authors: Irina Kusmartseva; Maria Beery; Helmut Hiller; Myriam Padilla; Stephen Selman; Amanda Posgai; Harry S. Nick; Martha Campbell-T...; Desmond A. Schatz; Michael J. Haller; Clive H. Wasserfall; Mark A. Atkinson
• Keywords: Enteroendocrine cell; Exocrine pancreas; Acinar cell

Within the human pancreas, exocrine and endocrine cells control secretion of digestive enzymes production hormones to maintain metabolic homeostasis, respectively. While vast majority type 1 diabetes research efforts have focused on function autoimmunity, recent studies identified a series unique features (e.g., reduced weight volume, increased density leukocytes) within pancreas in this disease, but mechanisms underlying these aberrancies are unknown. Therefore, we histologically assessed amylase, insulin, glucagon, lipase, and/or trypsinogen 78 organ donor pancreata from birth through adulthood subjects those at various stages diabetes. amylase-positive (AMY+) acinar were detectable all study groups, tissues individuals >2 years age contained clusters devoid amylase (AMY-). A AMY- cell localized proximal islets (i.e., peri-islet). Additionally, most positive for lipase trypsinogen. Interestingly, displayed significant reductions frequency clusters. These results support contribution islet-acinar axis pancreatic development underscore potential role pathogenesis