2155-P: Peripheral Nervous System Innervation of the Developing Pancreas

• DOI: 10.2337/db19-2155-p
• Publication Date: 2019-06-05T02:10:36Z
• Authors: SEAN DE LA O; LAUREN BYRNES; DANIEL M. WONG; SARAH KNOX; JULIE B. SNEDDON
• Keywords: Organogenesis; Peripheral Nervous System

Pancreatic organogenesis is a complex, multistep process whereby multiple cell types must migrate, proliferate, and differentiate in strict spatiotemporal manner to ultimately form the complete organ. Although pancreatic epithelial well characterized, peripheral nervous system (PNS) innervating organ poorly understood. PNS has been shown be critical formation of other budding exocrine organs, therefore likely plays role pancreas, there dearth information on innervation this developing To end, our lab taken advantage an explant culture single-cell RNA-sequencing (scRNAseq) characterize nerves supplying pancreas. Imaging whole pancreata reveals that develop parallel with intrapancreatic ganglia being present from early stages. Using scRNAseq analysis we have begun decipher timing progenitor differentiation, potential signaling pathways guiding axonal growth morphogenesis. Continued investigation mechanisms deciphering how nerve-epithelial interactions are established defining activated by will allow us discover morphogenesis, as mis-regulation during disease. Disclosure S. de la O: None. L. Byrnes: D.M. Wong: Knox: J.B. Sneddon: Advisory Panel; Spouse/Partner; Akouos. Self; Encellin. Consultant; Semma Therapeutics, Inc.