Metabolomic Profiling of Fatty Acid and Amino Acid Metabolism in Youth With Obesity and Type 2 Diabetes

• DOI: 10.2337/dc11-1577
• Publication Date: 2012-01-21T07:57:38Z
• Authors: Stephanie J. Mihalik; Sara F. Michaliszyn; Javier de las Heras; Fida Bacha; SoJung Lee; Donald H. Chace; Victor R. DeJesus; Jerry Vockley; Silva A. Arslanian
• Keywords: Male; 0301 basic medicine; 2. Zero hunger; 0303 health sciences; Adolescent; Fatty Acids; Fasting; 3. Good health; 03 medical and health sciences; Absorptiometry, Photon; Diabetes Mellitus, Type 2; Carnitine; Humans; Female; Obesity; Amino Acids; Original Research

We compared acylcarnitine (AcylCN) species, common amino acid and fat oxidation (FOX) byproducts, plasma acids in normal weight (NW; n = 39), obese (OB; 64), type 2 diabetic (n 17) adolescents.Fasting was analyzed by tandem mass spectrometry, body composition dual energy X-ray absorptiometry computed tomography, total-body lipolysis substrate [(2)H(5)]glycerol indirect calorimetry, respectively. In vivo insulin sensitivity (IS) assessed with a 3-h hyperinsulinemic-euglycemic clamp.Long-chain AcylCNs (C18:2-CN to C14:0-CN) were similar among the three groups. Medium- short-chain (except C8 C10) significantly lower diabetes NW, when OB, C2-, C6-, C10-CN lower. Amino concentrations NW. Fasting FOX higher OB negative association of C10:1 disappeared after controlling for adiposity, Tanner stage, sex. IS positive associations between arginine, histidine, serine adjusting sex.These metabolomics results, together increased rates FOX, are not supportive defective fatty or metabolism obesity youth. Such observations consistent early adaptive metabolic plasticity youth, which over time-with continued aging-may become dysfunctional, as observed adults.