Glucagon-Like Peptide 1 Attenuates the Acceleration of Gastric Emptying Induced by Hypoglycemia in Healthy Subjects

• DOI: 10.2337/dc13-1813
• Publication Date: 2014-03-06T03:34:39Z
• Authors: Mark P. Plummer; Karen L. Jones; Chris E. Annink; Caroline E. Cousins; Juris J. Meier; Marianne J. Chapman; Michael Horowitz; Adam M. Deane
• Keywords: Glucose clamp technique; Crossover study

Exogenous GLP-1 slows gastric emptying in health and diabetes leading to diminished glycemic excursions. Gastric is markedly accelerated by hypoglycemia. The primary objective was determine whether attenuates the acceleration of induced hypoglycemia.Ten healthy volunteers were studied on four separate days a randomized double-blind fashion. Blood glucose stabilized using glucose/insulin clamp at hypoglycemia (2.6 mmol/L two occasions [hypo]) or euglycemia (6.0 [eu]) between T = -15 45 min before clamping 6.0 until 180 min. During euglycemia, subjects received intravenous (1.2 pmol/kg/min) placebo. At 0 min, ingested 100 g beef mince labeled with 20 MBq (99m)Tc-sulfur-colloid 3 3-O-methyl-glucose (3-OMG), marker absorption. measured scintigraphically from serum 3-OMG taken 15-min intervals. areas under curve for concentration analyzed one-way repeated-measures ANOVA Bonferroni-Holm adjusted post hoc tests.Gastric during (hypo/placebo vs. eu/placebo; P < 0.001), as absorption (P 0.03). slowed (eu/placebo eu/GLP-1; 0.001). However, hypoglycemia-induced placebo hypo/GLP-1; 0.008), 0.01).Acute administration exogenous attenuates, but does not abolish, insulin-induced subjects.