Decrease in Insulin Receptors During Friend Erythroleukemia Cell Differentiation

• DOI: 10.2337/diab.28.9.823
• Publication Date: 2013-09-19T17:13:09Z
• Authors: Barry H Ginsberg; Thomas Brown; Mohan Raizada
• Keywords: Cooperative binding

The Friend erythroleukemia cell has an insulin receptor with all the properties of mammalian receptors: rapid, reversible, and saturable binding insulin; specific for analogs; inversely proportional to temperatures; sharply pH dependent (optimum = 8.0); demonstrated ligand-induced accelerated dissociation consistent negative cooperativity. There were 17,200 sites per cell. After induction by dimethylsulfoxide, 80% cells became benzidine positive (i.e., contained hemoglobin). concentration dropped 4300 cell, while remaining receptors retained initial characteristics. This loss could not be attributed directly either dimethylsulfoxide or changes in size. Thus, during process differentiation, decreases.