Insulinlike Activity of New Antidiabetic Agent CP 68722 in 3T3-L1 Adipocytes

• DOI: 10.2337/diab.39.11.1414
• Publication Date: 2013-09-19T17:22:06Z
• Authors: David K Kreutter; Kim M Andrews; E Michael Gibbs; Nancy J Hutson; Ralph W Stevenson
• Keywords: Deoxyglucose; 3T3-L1; Troglitazone

We examined the in vitro effects of CP 68722, a novel antidiabetic agent, 3T3-L1 adipocytes. 68722 stimulated 2-deoxyglucose uptake absence insulin. At least 30 min incubation were required for stimulation uptake. This effect increased over 5 h and was sustained up to 72 h. The by could be inhibited ∼60% inhibition protein synthesis with cycloheximide. Half-maximal maximal responses at observed 10 100 μM drug, respectively, threefold approximating response these cells acute insulin stimulation. able overcome resistance induced dexamethasone cells. like that insulin, primarily increase Vmax or prevented incubating 10°C, temperature impedes translocation glucose transporters plasma membrane. Therefore, it appears stimulates mechanism involves intracellular membrane de novo synthesis. compared sulfonylureas, primary drugs used treatment non-insulin-dependent diabetes mellitus (NIDDM). more potent effective stimulator than either first- second-generation sulfonylureas. Our results suggest an therapeutic agent NIDDM.