Evidence Against Altered Expression of GLUT1 or GLUT4 in Skeletal Muscle of Patients With Obesity or NIDDM

• DOI: 10.2337/diab.39.7.865
• Publication Date: 2013-09-19T17:22:06Z
• Authors: Oluf Pedersen; Jens F Bak; Per H Andersen; Sten Lund; David E Moller; Jeffrey S Flier; Barbara B Kahn
• Keywords: GLUT4; Streptozocin

Studies of experimental diabetes in rodents induced by the beta-cell toxin streptozocin have shown that insulin-resistant glucose transport peripheral tissues (muscle and adipose) these animals can be ascribed part to a pretranslational reduction major insulin-sensitive transporter (GLUT4) tissues. Because central feature non-insulin-dependent mellitus (NIDDM) is an imparied ability insulin enhance disposal skeletal muscle, we examined hypothesis reduced expression GLUT4 characteristic finding muscle subjects with NIDDM. Biopsies muscles were obtained from 17 patients NIDDM 10 lean 9 obese nondiabetic subjects. Among diabetic subjects, 7 newly diagnosed untreated. Compared age-matched body-weight-matched healthy control there was no significant alteration level mRNA demonstrated Northern blot slot or protein determined immunoblotting membranes. Neither nor concentration correlated degree glycemic control, fasting plasma glucose, duration, body mass index, sex, age. GLUT1 levels also not significantly different between matched Thus, unlike streptozocin-induced rodents, evidence impaired insulin-responsive responsible for moderately patients.