Reevaluation of Autoantibodies to Islet Cell Membrane in IDDM: Failure to Detect Islet Cell Surface Antibodies Using Human Islet Cells as Substrate

• DOI: 10.2337/diab.41.12.1624
• Publication Date: 2013-09-19T17:22:06Z
• Authors: Marta Vives; Nuria Somoza; Gloria Soldevila; Ramon Gomis; Anna Lucas; Anna Sanmarti; Ricardo Pujol-Bor...
• Keywords: Insulitis

Since their demonstration in 1975, ICSAs have been proposed as serological markers and pathogenic elements IDDM. are detected the sera of most newly diagnosed IDDM patients by indirect IFL that uses viable preparations rat islet or insulinoma cells substrate, but they also can be using human fetal cells. We tried to demonstrate 31 diabetic patients, including 6 positive samples on cells, which used natural target for first time: normal In spite different types these (i.e., freshly dispersed cell suspensions, monolayer cultures, islets after culture), could not under UV microscope, nor flow cytometry. contrast, 9 29 gave a staining RIN an attempt establish whether putative ICSA autoantigen is present surface pancreas, insulitis microenvironment was emulated exposing three stress: 1) cytokines (IFN-γ TNF-α); 2) heat shock; 3) hyperglycemia. However, failed again recognize membrane antigens either treatments. Neither were from patient stained its autologous serum (ICA titer >80 JDF U). These results suggest expressed therefore raises doubts about role.