1alpha,25-dihydroxyvitamin D3 induces an autoantigen-specific T-helper 1/T-helper 2 immune shift in NOD mice immunized with GAD65 (p524-543).
NOD mice
DOI:
10.2337/diabetes.49.8.1301
Publication Date:
2007-03-06T19:04:22Z
AUTHORS (9)
ABSTRACT
Prevention of type 1 diabetes in NOD mice by 1,25-dihydroxyvitamin D3 [1alpha,25(OH)2D3] is accompanied a T-helper (Th) 1/Th2 cytokine shift the pancreas. The aim this study was to investigate whether immune also occurs outside pancreas and it limited autoantigen-specific responses. treated with 1alpha,25(OH)2D3 (5 microg/kg every 2 days) or control vehicle were immunized GAD65 (p524-543) ovalbumin (OVA) rear footpads. First, we examined T-cell proliferation production (via enzyme-linked immunosorbent assay) draining lymph node cells vitro without peptide rechallenge. Although no differences measured between 1alpha,25(OH)2D3-treated after rechallenge, marked secretion profile seen mice: interleukin-4 increased (37 +/- 5 vs. 21 12 pg/ml controls, P < 0.005), whereas gamma-interferon levels decreased (6 3 9 ng/ml 0.05). This absent OVA-primed mice. Second, profiles reverse transcriptase-polymerase chain reaction popliteal nodes at different time points priming OVA vivo. A Th1/Th2 occurred vivo GAD65. Again, immunization. Finally, rechallenge panel autoantigens (GAD65, heat shock protein 65, insulin B-chain) antigens (OVA, keyhole limpet hemocyanine, myelin proteolipid protein, tetanus toxin) confirmed autoantigen-injected but not antigen-injected In conclusion, deviation induced can be peripheral system pancreatic autoantigens.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (0)
CITATIONS (135)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....