Tissue Gene Expression of Renin-Angiotensin System in Human Type 2 Diabetic Nephropathy

• DOI: 10.2337/diacare.29.04.06.dc05-1873
• Publication Date: 2006-05-10T22:42:37Z
• Authors: Tadashi Konoshita; Shigeyuki Wakahara; Shinichi Mizuno; Makoto Motomura; Chikako Aoyama; Yasukazu Makino; Yasuyuki Kawai; Norihiro Kato; Ichiro Koni; Isamu Miyamori; Hiroshi Mabuchi
• Keywords: Adult; Male; 0301 basic medicine; Angiotensins; Gene Expression; Glyceraldehyde-3-Phosphate Dehydrogenases; Receptors, Cell Surface; Middle Aged; Peptidyl-Dipeptidase A; Kidney; Polymerase Chain Reaction; Up-Regulation; 3. Good health; Renin-Angiotensin System; Proteinuria; 03 medical and health sciences; Diabetes Mellitus, Type 2; Renin; Humans; Diabetic Nephropathies; Female; Kidney Diseases; RNA, Messenger

OBJECTIVE—Recent studies have proved that blockade of the renin-angiotensin system (RAS) retards progression diabetic nephropathy, whereas hyporeninemia is known as a typical state in subjects. The purpose this study to determine whether expression levels RAS differ between nondiabetic and renal tissues with accurate quantitative method. RESEARCH DESIGN AND METHODS—Subjects were 66 8 patients biopsy-proven diseases. eight subjects suffered from type 2 diabetes overt proteinuria. Renal histology revealed diffuse or nodular lesions linear IgG deposit on immunofluorescent staining thickened basement membrane electronic microscopy. Total RNA small part cortical biopsy specimens was reverse-transcribed, resultant cDNA amplified for new major components (i.e., renin, renin receptor, angiotensinogen, ACE, ACE2, angiotensin II 1 receptor) measured. RESULTS—Among these components, significant upregulation observed ACE gene tissue. CONCLUSIONS—The results suggest tissue might be activated respect upregulated spite tendency low nephropathy.