<b>Optimised proteomic analysis of insulin granules from MIN6 cells identifies Scamp3, a novel regulator of insulin secretion and content.</b>

• DOI: 10.2337/figshare.27091654.v1
• Publication Date: 2024-09-25T15:51:47Z
• Authors: Nicholas Norris; Belinda Yau; Carlo Famularo; Hayley Webster; Thomas Loudovaris; Helen E. Thomas; Mark Larance; Alistair M. Senior; Melkam A. Kebede
• Keywords: Negative regulator

&lt;p dir="ltr"&gt;&lt;b&gt;Abstract&lt;/b&gt;&lt;/p&gt;&lt;p dir="ltr"&gt;Pancreatic b-cells in the &lt;i&gt;Islets of Langerhans&lt;/i&gt; are key to maintaining glucose homeostasis, by secreting peptide hormone insulin. Insulin is packaged within vesicles named insulin secretory granules (ISGs), that have recently been considered intrinsic structures and proteins regulate granule maturation, trafficking, secretion. Previously, studies identified a handful novel ISG-associated using different separation techniques. Here, this study combines an optimized ISG isolation technique mass spectrometry-based proteomics, with unbiased protein correlation profiling targeted machine learning approach uncover 211 confidence. Four these proteins: Syntaxin-7, Synaptophysin, Synaptotagmin-13 Scamp3 not previously ISG-associated. Through colocalization analysis confocal imaging we validate association MIN6 human b-cells. We further role for one (Scamp3) regulating content secretion from first time. knock-down INS-1 cells show reduction dysfunctional These data provide basis future investigation b-cell biology regulation secretion.&lt;/p&gt;&lt;p dir="ltr"&gt;&lt;b&gt;Keywords&lt;/b&gt;: granule, beta-cell, pancreas, syntaxin-7, synaptophysin, synaptotagmin-13, scamp3&lt;/p&gt;&lt;p&gt;&lt;br&gt;&lt;/p&gt;&lt;p dir="ltr"&gt;&lt;b&gt;Article Highlights&lt;/b&gt;&lt;/p&gt;&lt;p dir="ltr"&gt;· This optimizes techniques alongside enhanced proteomics analyses establish murine proteome.&lt;/p&gt;&lt;p investigated what present on our understanding biogenesis, found granule-associated validated 4 proteins.&lt;/p&gt;&lt;p functional studies, implicated as regulates b-cells.&lt;/p&gt;