Hepatoblastomas and Liver Development: A Study of Cytokeratin Immunoexpression in Twenty-Nine Hepatoblastomas

• DOI: 10.2350/05-12-0002.1
• Publication Date: 2006-07-12T21:06:12Z
• Authors: Mariana Morais Ca...; José Ivanildo Neves; Fabiana Facco Cas...; Beatriz de Camargo; Paulo ChapChap; Simone Treiger Sr...; Fernando Augusto ...
• Keywords: Male; Carcinoma, Hepatocellular; Liver Neoplasms; Infant, Newborn; Infant; Immunohistochemistry; 03 medical and health sciences; 0302 clinical medicine; Liver; Child, Preschool; Humans; Keratins; Vimentin; Female; alpha-Fetoproteins; Child; Oligonucleotide Array Sequence Analysis; Retrospective Studies

Hepatoblastomas (HBs) recapitulate liver development. It is possible that HBs result from malignant transformation of hepatic precursor cells, and they may reflect a blockage in normal Here we study the expression cytokeratins (CKs) order to delineate immunoprofile relationship with development, as well vimentin alphafetoprotein (AFP), HBs. Immunohistochemistry was performed tissue microarray (TMA) containing representative areas 18 (fetal and/or embryonal mesenchymal); also reviewed 11 cases not included TMA. No stained for CKs 1, 5/6, 7, 10, 13, 15, 16, 20, 34βE12. CK8 73.07% fetal, 50% embryonal, 18% mesenchymal areas. CK18 100% epithelial CK19 staining intense diffuse samples, but it weaker fetal (66.66%). AE1 all cases, 29.41% AE3 84.61% 60% components. AE1/AE3 showed stronger (100%) than (76.92%). Vimentin strong (66.66%) (84.61%) components weak (8%). Alphafetoprotein positive only 20% 70% Our results support hypothesis immunoexpression follows stages Embryonal look less differentiated, expressing biliary epithelium CKs, whereas display more developed phenotype, similar mature hepatocytes. These data aid understanding ontogenesis be used histopathological diagnosis.