Novel Karyotypes and Cyclin D1 Immunoreactivity in Clear Cell Sarcoma of the Kidney

• DOI: 10.2350/14-12-1581-oa.1
• Publication Date: 2015-03-09T21:38:42Z
• Authors: Sze Jet Aw; Chik Hong Kuick; Min Hwee Yong; Derrick Wen Quan ...; Shi Wang; Alwin Hwai Liang Loh; Sharon Ling; Guat Lian Peh; Shui Yen Soh; Amos Hong Pheng Loh; Puay Hoon Tan; Kenneth Tou En Chang
• Keywords: Tissue microarray

Pathological diagnosis of clear cell sarcoma the kidney (CCSK) is challenging as it resembles blastemal Wilms tumor (WT) and other pediatric sarcomas, does not have any distinctive immunophenotype. The YWHAE-FAM22 translocation t(10;17)(q22;p13) has been reported in a subset CCSK. This also occurs high-grade endometrial sarcoma, which associated with cyclin D1 overexpression. Hence we seek to determine status immunoreactivity series local CCSK cases. Of 8 cases from 7 patients identified, no had fusion transcript by reverse transcriptase–polymerase chain reaction. Novel karyotypes were identified for 2 cases: 1 t(2;13)(q13; q22) t(3:17)(q29;p11.2). Excluding case poor tissue section antigenicity, CCSKs (100%) showed diffuse strong nuclear staining. Cyclin immunohistochemistry was performed on microarrays renal tumors: areas 18 WT negative; 6 rhabdoid tumors metanephric adenoma patchy weak staining; 3 mesoblastic nephromas 29 neuroblastomas positive helps distinguish tumors, but nephromas. overexpression contingent translocation, inhibition may potentially be explored targeted therapeutic strategy