Unveiling the functional and evolutionary landscape of RNA editing in chicken using genomics and transcriptomics

• DOI: 10.24272/j.issn.2095-8137.2022.331
• Publication Date: 2022-10-19T11:34:33Z
• Authors: Yun-Mei Wang; ; ; Ling-Qun Ye; Ming-Shan Wang; Jin-Jin Zhang; Saber Khederzadeh; David M Irwin; Xiao-Die Ren; Ya-Ping Zhang; Dong-Dong Wu
• Keywords: ADAR; Inosine; Riboswitch; Functional Genomics

The evolutionary and functional features of RNA editing are well studied in mammals, cephalopods, insects, but not birds. Here, we integrated transcriptomic whole-genomic analyses to exhaustively characterize the expansive repertoire adenosine-to-inosine (A-to-I) sites (RESs) chicken. In addition, investigated status chicken editome as a potential mechanism domestication. We detected lowest level liver chickens, compared muscles humans, found higher activity specificity brain than non-neural tissues, consistent with brain’s complexity. To certain extent, specific may account for functions tissues. Our results also revealed that sequences critical RES secondary structures remained conserved within avian evolution. Furthermore, was shaped by purifying selection during domestication most RESs have served pool few involved domestication, including evolution nervous immune systems. Regulation chickens adenosine deaminase acting on (ADAR) enzymes be affected non-ADAR factors whose expression levels changed widely after <i>ADAR</i> knockdown. Collectively, provide comprehensive lists candidate non-ADAR-editing regulators chicken, thus contributing our current understanding animals.