Cross-species recognition of bat coronavirus RsYN04 and cross-reaction of SARS-CoV-2 antibodies against the virus

• DOI: 10.24272/j.issn.2095-8137.2023.187
• Publication Date: 2023-09-12T22:02:06Z
• Authors: Runchu Zhao; ; ; Sheng Niu; Pu Han; Yue Gao; Dezhi Liu; Chunliang Luo; Honghui Liu; Bo Liu; Yanli Xu; Jianxun Qi; Zhihai Chen; Weifeng Shi; Lili Wu; George F. Gao; Qihui Wang
• Keywords: Coronavirus; Lineage (genetic)

Since the outbreak of COVID-19, many SARS-CoV-2-related coronaviruses (CoVs) have been discovered. Previous research identified a novel lineage CoVs in bat, such as RsYN04, which recognizes human angiotensin-converting enzyme 2 (ACE2) and poses potential threat to humans. Here, we screened binding RsYN04 receptor-binding domain (RBD) ACE2 orthologs from 52 animal species, found that virus shows narrower ACE2-binding spectrum than SARS-CoV-2. However, T484W mutation RBD can broaden spectrum. We also evaluated 44 SARS-CoV-2 antibodies targeting seven epitope communities RBD, together with sera COVID-19 convalescents vaccinees, for their cross-reaction against RsYN04. Our results showed all antibodies, except RBD-6 RBD-7 classes, did not bind indicating substantial immune difference Furthermore, complex structure cross-reactive antibody S43 revealed potently broad development therapeutics vaccines. findings suggest other viruses belonging same clade infect several species including humans, highlighting necessity viral surveillance anti-coronavirus countermeasures.