Effects of interleukin-6 and interleukin-1β on expression of growth differentiation factor-5 and Wnt signaling pathway genes in equine chondrocytes

• DOI: 10.2460/ajvr.75.2.132
• Publication Date: 2014-01-28T17:52:20Z
• Authors: Emilia Svala; Anna I. Thorfve; Cecilia Ley; Helena K. Barreto...; Jane M. Synnergren; Anders H. Lindahl; Stina Ekman; Eva S. R. Skiölde...
• Keywords: Growth differentiation factor; Immunostaining

Abstract Objective —To determine the effects of interleukin (IL)-6 and IL-1β stimulation on expression growth differentiation factor (GDF)-5 Wnt signaling pathway genes in equine chondrocytes. Sample —Macroscopically normal articular cartilage samples from 6 horses osteochondral fragments (OCFs) 3 horses. Procedures —Chondrocyte pellets were prepared cultured without or following with IL-6 for 1, 2, 12, 48 hours; GDF-5 was determined a quantitative real-time PCR assay. Expression various pathways microarrays stimulated 1 2 hours. Immunohistochemical analysis used to detect GDF-5, glycogen synthase kinase 3β (GSK-3β), β-catenin proteins macroscopically OCFs. Results —Chondrocytes had significantly higher within hours versus unstimulated Microarray indicated GSK-3β coiled-coil domain containing 88C increased after hour decreased stimulation. immunohistochemical detection similar microarray results. Chondrocytes OCFs immunostaining GDF-5. Conclusion Clinical Relevance —Results chondrocyte canonical transactivator β-catenin, induced inhibitors pathway, This suggested may inhibit subsequent upregulation expression. Anabolic extracellular matrix metabolism be attributable information could useful development repair methods.