Determination of the antibacterial effect of bee venom against rainbow trout pathogens and antibiotic resistance gene expression

• DOI: 10.2478/acve-2023-0028
• Publication Date: 2023-09-28T17:13:36Z
• Authors: Dilek Kabakci; Çiğdem Ürkü; Şükrü Önalan
• Keywords: Mass Fragmentography; Veterinary medicine; Antibiotic Agent; Honeybee; Bacterium Colony; Gene Expression; Oxytetracycline; Aquaculture; Disk Diffusion; antibacterial activity; SF600-1100; Pathogenic Fish Bacteria; Fluorometry; Infectious Agent; 0303 health sciences; Hly Gen Expression; Agar Diffusion; bee venom; Bacterial Gene; Minimum Bactericidal Concentration; Minimum Inhibitory Concentration; Biological Pest Control; Bee Venom; Lactococcus Garvieae; Real Time Polymerase Chain Reaction; Hemolysin; Oncorhynchus Mykiss; honeybee; Apis Mellifera; Antimicrobial Activity; Article; Propolis; Fibronectin Binding Protein; 03 medical and health sciences; Rna Isolation; Bacterial Strain; Vibrio Anguillarum; Yersinia Ruckeri; Controlled Study; Mortality; Fibronectin; Aeromonas Hydrophila; Gas Chromatography; hly gen expression; Bacteria; Bacterial Growth; Probiotics; Rna Extraction; Fate; Nonhuman; pathogenic fish bacteria; Antibacterial Activity; Aeromonas-Hydrophila; Antibiotic Resistance; Zone Of Inhibition; Gene Expression Level

Abstract Bee venom (BV) is a rich source of secondary metabolites from honeybees (Apis mellifera L.). It contains a variety of bioactive ingredients including peptides, proteins, enzymes, and volatile metabolites. This study investigated the antibacterial effects of the bee venom obtained from honey bees (Apis mellifera L.) against bacterial fish pathogens, such as Lactococcus garvieae (Lg1, Lg2, Lg3), Vibrio anguillarum (Va1, Va2, Va3), Yersinia ruckeri (Yr1, Yr2, Yr3), and Aeromonas hydrophila (Ah1, Ah2, Ah3) and the expression levels on the antibiotic resistance genes hly and fbp (hemolysin and fibronectin-binding prot) of them. It was determined that bee venom had an antibacterial effect against L. garvieae, L. anguillarum, and Y. ruckeri strains, while it had no effect only against Ah3 and Ah2 bacterial strains. As staded by the gene expression of hly (hemolysin) and fbp (fibronectin-binding protein), among the antibiotic resistance genes the effect levels of bee venom on bacterial species varied, although it affected antibiotic resistance and gene expression level in all bacteria. It was revealed that the expression level was the highest for V. anguillarum strains, whereas it was below the control group for L. garvieae. i.e the effect of bee venom on the resistance mechanism for L. garvieae was much less compared to V. anguillarum. Based on the results in the current study it could be concluded that applying bee venom to pathogenic bacteria that cause mortality in the aquaculture sector could induce the defense-related gene and change the broad-spectrum biocontrol activity at the molecular level.