Potential Gastric Cancer Immunotherapy: Stimulating the Immune System with Helicobacter pylori pIRES2-DsRed-Express-ureF DNA Vaccines

• DOI: 10.2478/aite-2024-0004
• Publication Date: 2024-02-12T19:42:12Z
• Authors: Mahsa Afkhamipour; Fatemeh Kaviani; Samaneh Dalali; Tohid Piri-Gharaghie; Abbas Doosti
• Keywords: 0301 basic medicine; 0303 health sciences

Abstract Most gastric cancers (GC) are thought to be caused by Helicobacter pylori ( H. ) infections. However, there is mounting evidence that GC patients with positive status have improved prognoses. The -induced cellular immune reaction may inhibit cancer. In this study, BALB/c mice were immunized using recombinant plasmids encode the ureF gene of . Purified functional splenic CD3 + T lymphocytes used study anticancer effects in vitro and vivo immunological state ongoing infection mimicked DNA vaccines, which cause a change from Th1 Th2. Human cells grow more slowly when stimulated as adoptive infusions because they reduce xenograft development excellent ratios infiltrating CD8 /CD4 cells, decreased invasion regulatory FOXP3 Treg lymphocytes, increased apoptosis brought on Caspase9/Caspase-3 overexpression Survivin downregulation all contribute consequences. Our findings suggest people advanced GC, pIRES2-DsRed-Express- vaccines immunotherapeutic utility.