Effects of Propofol on the Formation and Activity of Osteoclast-Like Cells

• DOI: 10.2485/jhtb.33.155
• Publication Date: 2024-07-19T23:45:50Z
• Authors: Hitomi Satomi; Takayuki Kawato; Hideki Tanaka; Kumiko Nakai; Takeo Sugita; Shouhei Ogisawa; Shuichi Nishikubo; Shunichi Oka; Hirofumi Arisaka
• Keywords: Cathepsin K

Propofol affects the central nervous system rapidly and works as an anesthetic. After intravenous administration, cells resident in bone tissues are also exposed to propofol. Osteoclasts can form actin rings on surfaces express carbonic anhydrase II (Car2), cathepsin K (Ctsk), matrix metalloproteinase-9 (Mmp9), which enzymes associated with decomposition play a role resorption. This study examined expression of involved resorption ring formation stimulated by propofol elucidate difference effects continuous temporary stimulation osteoclast ability. RAW264.7 were emulsified injection solution or 2,6-diisopropylphenol (non-emulsified propofol) for 5 h, 1.5 days, 4 days presence receptor activator nuclear factor kappa B ligand (RANKL). Osteoclast-like confirmed staining tartrate-resistant acid phosphatase (TRAP). Actin detected using fluorescent staining. The resorp­tion real-time PCR Western blotting. mineral activity was assessed pit assay. Continuous more than suppressed Car2, Ctsk, Mmp9 expression. h had no suppressive effects, whereas prolonged (> days) decreased area pits. There marked versus non-emulsified TRAP-positive multinuclear cell formation. Our vitro indicated that exposure several hours did not affect their subsequent RANKL-induced differentiation into osteoclast-like cells, thus maintaining function. However, continuing cells.