Effects of High Glucose Concentrations on HMGB1 Expression in MG-63 Cells

• DOI: 10.2485/jhtb.33.23
• Publication Date: 2024-01-30T22:20:22Z
• Authors: Junya Nakajima; Kumiko Nakai; Hideki Tanaka; Manami Ozaki; Kyoko Fukuzawa; Takayuki Kawato; Yoshiyuki Yonehara
• Keywords: HMGB1; RAGE; Proinflammatory cytokine

Diabetes is a metabolic disorder that causes long-term hyperglycemic state with complications affect multiple tissues, including bone. HMGB1, nonhistone chromosomal binding protein, released from the nucleus of damaged cells and secreted extracellularly, where it mediates inflammatory responses. Hyperglycemia induces HMGB1 expression in cell types associated diabetic complications. Therefore, we evaluated effect high glucose concentration on production MG-63 osteoblast-like cells. were cultured presence at 5.5 mM (control) or 25.0 (high glucose). The mRNA levels receptors (RAGE, TLR2, TLR4), HSP90AA1, cytokines (IL-6 TNF-α) analyzed by quantitative PCR. protein TNF-α ELISA, immunofluorescence staining, Western blotting. RAGE, TLR4, TNF-α, IL-6 higher group than control group. Also, upregulated level. Anti-HMGB1 antibodies partially blocked glucose-induced increase but not IL-6, level was also decreased anti-HMGB1 antibodies. In conclusion, induced secretion cells, suggesting extracellular osteoblasts under conditions.