NAT10 Promotes Malignant Progression of Lung Cancer via the NF-κB Signaling Pathway

• DOI: 10.24976/discov.med.202335179.89
• Publication Date: 2023-12-01T08:44:27Z
• Authors: Xia Liu; Xinyun Liu; Chaoshi Zhuo; Jiangfeng Shen; Kaijin Lu; Min Sha; Jun Ye; Junxing Huang; Hexu Han; Hong Yu
• Keywords: Acetyltransferases

Background: NAT10 (N-acetyltransferase 10) is a newly identified novel acetyltransferase. Abnormal expression of associated with several human disorders, including cancer, autoimmune diseases, and cardiovascular disease. This study aimed to investigate the role in promoting lung cancer malignant progression through NF-κB (nuclear factor κB) signaling pathway. Methods: Cells lines BEAS-2B, NCI-H524, A549, PC-9, NCI-H23, NCI-H258 were cultured for identification. Western blotting PCR assays determined gene within sample cells. Cellular functionality was assayed using CCK8 (Cell Counting Kit-8), Dual-Luciferase Reporter, Colony formating. Results: The assay showed significant elevation levels tumor tissues compared paraneoplastic (<i>p</i> < 0.05). Specifically, only affected content RelA/p65 cancer. Analysis from TCGA (The Cancer Genome Atlas) database indicated that elevated tumors can be good prognostic indicator patients. knockdown significantly suppressed A549 cells' rate colony formation further confirmed overexpression increased generation clones NCI-H524 cells proliferation influenced by inhibited blocking pathway Dual-luciferase reporter results revealed NAT10's potential pathway's activity Immunohistochemical staining underscored strong link between protein tissues. Conclusions: upregulated tissues, supported results. plays development activate Hence, regulation critical