Architecture and Functional Analysis of Fungal Polyketide Synthases

• DOI: 10.2520/myco.52.135
• Publication Date: 2009-01-08T06:00:32Z
• Authors: Isao FUJII; Akira WATANABE; Hidenori TADA; Yuya ONO; Yuichiro MORI; Sayaka KAJIMOTO; Yoshinori YASUOKA; Yutaka EBIZUKA; Ushio SANKAWA
• Keywords: Aspergillus oryzae; Aspergillus nidulans; Aspergillus terreus; Polyketide synthase; Heterologous

All fungal polyketide synthase (PKS) genes so far cloned code for iterative type I PKSs that are different from bacterial modular and II PKSs. In order to clarify how control their reactions produce specific product compounds, such as Aspergillus terreus ATX, nidulans WA, Colletotrichum lagenarium PKS1, fumagatus Alb1p derivatives, were expressed under α-amylase promoter in heterologous host oryzae. Chemical identification of products confirmed functions gave some insights into controlled.