Effect of a short-term fast on intestinal disaccharidase activity and villus morphology of piglets suckling insulin-like growth factor-I transgenic sows1

• DOI: 10.2527/2005.83102404x
• Publication Date: 2016-08-09T17:19:59Z
• Authors: J. L. Hartke; M. H. Monaco; M. B. Wheeler; S. M. Donovan
• Keywords: Disaccharidase; Jejunum

The objectives of this study were to use transgenic sows that overexpress IGF-I in milk investigate the effect a short-term fast on piglet intestinal morphology and disaccharidase activity determine how milk-borne influences response fasting. After farrowing, litters normalized 10 piglets. On d 6, piglets (n = 30) suckling (TG) nontransgenic (control) assigned randomly three treatments: fed (0 h), which remained with sow until euthanized 7, or fasted piglets, removed from at either 6 12 h before euthanasia 7. Serum IGFBP, weight length, jejunal protein DNA content, activity, villus measured. Fasting for resulted negative change between 7 (quadratic fasting; P < 0.001). Piglets TG tended have greater length (P 0.068), but no overexpression was noted weight. Fasting, however, linear 0.001) quadratic 0.002) decreases did not differ control sows, decreased linearly 0.003) IGFBP-4 (linear quadratic; ≤ 0.02) fasting, whereas IGFBP-1 increased quadratically Jejunal height, width, crypt depth all fasting 0.04). Disaccharidase affected by state; had lactase-phlorhizin hydrolase 0.01) sucrase-isomaltase activities than In summary, weight, morphology, serum IGF-I, -4, BW altered Thus, duration food deprivation euthanization should be considered when designing experiments assess development IGF axis, as magnitude differences state may exceed those expected result experimental treatment.