Angiotensin 1-7 formation in breast tissue is attenuated in breast cancer - a study on the metabolism of angiotensinogen in breast cancer cell lines.

• DOI: 10.26402/jpp.2019.4.02
• Publication Date: 2019-08-01
• Authors: Beata Bujak-Giżycka; J. Madej; Beata Bystrowska; Justyna Totoń‐Żur...; Krzysztof Kus; Maria Kołton‐Wróż; Jacek Jawień; Rafał Olszanecki
• Keywords: Proto-Oncogene Proteins; Angiotensinogen; Humans; Breast Neoplasms; Female; Breast; Angiotensin I; Proto-Oncogene Mas; Peptide Fragments; Cell Line; Receptors, G-Protein-Coupled

The tissue renin-angiotensin system (RAS) plays an important role in the development and progression of many diseases. It has been confirmed that angiotensin II (ANG II) participates proliferation angiogenesis breast cancer. Moreover, some RAS dysregulations cancer have observed. Recent studies on two opposite axes angiotensinogen metabolism - ACE (angiotensin-converting enzyme)/ANGII/AT1R (angiotensin receptor type 1) ACE-2/ANG 1-7/MAS (mitochondrial assembly) indicate their importance tumor growth invasion, but describing metabolic pathways newer angiotensins, such as ANG 1-12, remain lacking. In this study, fragments three lines, namely, MDA-MB-231, MCF-7, T-47D, compared with normal cells (PCS-600) was estimated. Incubation resulted prevalent formation 1-7. A difference ability to form observed between cell lines. cells, strong predominance pathway detected. differences depending line were observed; prevalence ACE/ANG II/AT1R shown. Expressions component dysregulated differed conclusion, produce numerous peptide metabolites demonstrated. various types cells. obtained results greater classical production 1-12 seems be marginal tissue, a tendency for higher 1-7 significantly lower whereas expression MAS than control. This finding suggests substances agonist activity could useful treatment cancer, requires further investigations.