m6A-mRNA methylation regulates cardiac gene expression and cellular growth

• DOI: 10.26508/lsa.201800233
• Publication Date: 2019-04-09T23:19:03Z
• Authors: Vivien Kmietczyk; Eva Riechert; Laura Kalinski; Etienne Boileau; Ellen Malovrh; Brandon Malone; Agnieszka Gorska; Christoph Hofmann; Eshita Varma; Lonny Jürgensen; Verena Kamuf-Schenk; Janine Altmüller; Rewati Tappu; Martin Busch; Patrick Most; Hugo A Katus; Christoph Dieterich; Mirko Völkers
• Keywords: Cardiomyopathy, Dilated; Male; 0303 health sciences; Adenosine; Methyltransferases; Cell Enlargement; Methylation; Rats; 3. Good health; Cohort Studies; Mice; 03 medical and health sciences; Gene Expression Regulation; Gene Knockdown Techniques; Protein Biosynthesis; Animals; Humans; Myocytes, Cardiac; RNA, Messenger; Technology Platforms; Research Articles; Cells, Cultured; Cell Proliferation; Cell Size

Conceptually similar to modifications of DNA, mRNAs undergo chemical modifications, which can affect their activity, localization, and stability. The most prevalent internal modification in mRNA is the methylation adenosine at N6-position (m6A). This returns a role as central hub information within cell, serving an carrier, modifier, attenuator for many biological processes. Still, precise such m6A human murine-dilated cardiac tissue remains unknown. Transcriptome-wide mapping allowed us catalog targets murine hearts. Increased was found cardiomyopathy. Knockdown overexpression writer enzyme Mettl3 affected cell size cellular remodeling both vitro vivo. Our data suggest that highly dynamic cardiomyocytes undergoing stress changes methylome regulate translational efficiency by affecting transcript Once elucidated, manipulations specific sites could be powerful approach prevent worsening function.