Robust repression of tRNA gene transcription during stress requires protein arginine methylation

• DOI: 10.26508/lsa.201800261
• Publication Date: 2019-06-03T16:39:13Z
• Authors: Richoo B Davis; Neah Likhite; Christopher A Jac...; Tao Liu; Michael C Yu
• Keywords: RNA polymerase II; Transcription; RNA polymerase III; Protein methylation

Protein arginine methylation is an important means by which protein function can be regulated. In the budding yeast, this modification catalyzed major methyltransferase Hmt1. Here, we provide evidence that Hmt1-mediated of Rpc31, a subunit RNA polymerase III, plays context-dependent roles in tRNA gene transcription: under conditions optimal for growth, it positively regulates transcription, and setting stress, promotes robust transcriptional repression. context Rpc31 allows its interaction with III global repressor Maf1. Interestingly, mammalian Hmt1 homologue able to methylate one Rpc31’s human homologue, RPC32β, but not paralogue, RPC32α. Our data led us propose efficient model whereby facilitates metabolic economy coordinates protein-synthetic capacity.