PI3P-dependent regulation of cell size and autophagy by phosphatidylinositol 5-phosphate 4-kinase

• DOI: 10.26508/lsa.202301920
• Publication Date: 2023-06-14T17:50:14Z
• Authors: Avishek Ghosh; Aishwarya Venugopal; Dhananjay Shinde; Sanjeev Sharma; Meera Krishnan; Swarna Mathre; Harini Krishnan; Sankhanil Saha; Padinjat Raghu
• Keywords: Phenocopy

Phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 5-phosphate (PI5P) are low-abundance phosphoinositides crucial for key cellular events such as endosomal trafficking autophagy. 4-kinase (PIP4K) is an enzyme that regulates PI5P in vivo but can act on both PI3P vitro. In this study, we report a role PIP4K regulating levels Drosophila . Loss-of-function mutants of the only gene show reduced cell size salivary glands. elevated dPIP4K 29 reverting back towards WT, without changes levels, rescue size. also up-regulation autophagy be reverted by depleting Atg8a required Lastly, increasing WT phenocopy reduction associated seen Thus, our study reports PIP4K-regulated pool control