Application of solid-phase extraction and gas chromatography-mass spectrometry (SPE-GC-MS) in resolution of metabolism pattern of higher alcohols in rat plasma

• DOI: 10.26599/fshw.2023.9250033
• Publication Date: 2024-02-26T08:11:14Z
• Authors: Yufei Liu; Xiaonian Cao; Zhilei Zhou; Qingxi Ren; Zhongwei Ji; Min Gong; Yuezheng Xu; Weibiao Zhou; Shuguang Chen; Jian Mao
• Keywords: Low resolution

Higher alcohols are key factors affecting sensory quality and post-drinking comfort of alcoholic beverages. A strategy combining solid-phase extraction gas chromatography-mass spectrometry (SPE-GC/MS) was established to analyze the metabolism pattern higher in rat plasma after gavage four common beverages including huangjiu, baijiu, wine brandy. Seven milliliter dichloromethane determined as optimal condition, eight were precisely quantified with detection limits 1.82-11.65 μg/L, recoveries 89.07%-110.89% fine repeatability. The fastest absorption elimination rates total observed baijiu huangjiu group, respectively, highest peak concentration found brandy group. Additionally, metabolic rate isoamyl alcohol group faster than that at same intragastric administration dosage. This study may provide potential insight for evaluation beverage quality.