Chlorantraniliprole exposure aggravates high-fat diet-induced metabolic disorders in mice by regulating gut microbiota and its metabolites

• DOI: 10.26599/fshw.2024.9250079
• Publication Date: 2024-05-10T02:40:22Z
• Authors: Ting Gu; Jie Lin; Jiani Yang; William Mumby; Quancai Sun; Ye Peng
• Keywords: metabolic disorder; untargeted metabolomics; obesity; gut microbiota; Nutrition. Foods and food supply; TX341-641; chlorantraniliprole

Food contaminants, particularly insecticides, are important factors contributing to obesity and other adverse effects. As the most widely used diamide insecticide worldwide, chlorantraniliprole (CP) is ubiquitous in food environment. However, influence of CP on gut microbiota remains unknown. In this study, we administered CP/carboxymethyl cellulose sodium C57BL/6J mice with a high-fat diet (HFD) via gavage for 13 weeks. The exposure induced significant increases body weight gain, fat mass, serum total cholesterol (TC), low-density lipoprotein (LDL-C). Moreover, aggravated imbalance by increasing relative abundance obesity-associated bacteria reducing beneficial bacteria. Based untargeted metabolomics analysis, was found be involved regulation pathways including 'alanine, aspartate glutamate metabolism' 'arginine proline metabolism'. Notably, primarily alterations microbial metabolites within these pathways, such as <em>L</em>-(+)-aspartic acid <em>L</em>-glutamic acid. Additionally, individual metabolites, lipid mediator (oleoyl ethanolamide), also demonstrated upon exposure. Furthermore, Spearman correlation analysis revealed several noteworthy associations between alterations, metabolite changes, phenotypes. results study demonstrate connection microbiota, effects HFD-induced obesity, elucidating critical role its toxic CP.