Sequencing and Detection of Two Missense Variants at Chromosome 7 as Potential Azole Resistance Markers in Saccharomyces Cerevisiae

• DOI: 10.26685/urncst.363
• Publication Date: 2022-07-28T15:15:27Z
• Authors: Michael Trinh
• Keywords: Clotrimazole; Azole; Antifungal drugs

Introduction: Widespread practices of over-prescribing antibiotic, antiviral, and antifungal drugs have sparked concern over the risk antimicrobial resistance arising in bacterial, viral, fungal pathogens. This threatens to jeopardize efficacy many being prescribed today for said infections. Azoles are a major class drugs, presenting need research efforts on mechanisms azole resistance. My objective was perform genomic sequencing variant profiling Baker’s Yeast (Saccharomyces cerevisiae) strain that displays phenotype when plated with Clotrimazole, type antifungal. Methods: Through short-read subsequent calling, putative genotypes were elucidated clotrimazole-resistant yeast strain. Results: Variant calls at PDR1 ERG25 loci reveal two potential Clotrimazole-resistance genotypes. Discussion: These variants both missense mutations involving single-nucleotide change reference question. Although markers yeast, emergence novel may contribute this has not yet been. Conclusion: Follow-up experiments will include induced selection assays confirm described here indeed constitute Establishing knowledgebase marker genes model organisms such as Saccharomyces cerevisiae can guide investigations into orthologous proteins other species become responsible future antifungal-resistant