Surgical Technique of Small Bowel Transplantation in a Large Animal Model

• DOI: 10.2754/avb201079020281
• Publication Date: 2010-08-11T17:59:53Z
• Authors: Martin Oliverius; Dušan Král; Eva Honsová; Alena Lodererová; Michal Kudla; Petr Baláž; Alexandros Valsamis; Jiří Čáp
• Keywords: Immunosuppression; Allotransplantation; Animal model

An experiment was carried out on pigs during preparation of a clinical project intestinal transplantation. The objective this study to find the best surgical technique vascular and anastomosis in different experimental settings (animals with without immunosuppression) which could have major impact everyday veterinary practice. Transplantation performed 43 pigs. In part we examined most suitable anastomosis. A running continuous single-layer seromuscular suture used for all types anastomoses. With regard anastomosis, animals were divided into two groups. group 1 (n = 18) one animal both donor recipient graft. Anastomoses constructed mesenteric vessel bed. 2 25), graft another recipient, revascularization central second study, immunosuppressive drug administration acute cellular rejection survival. Animals that died due technical failure reasons unrelated transplantation excluded from evaluation (19 pigs). total 24 included study. four Group - autotransplantation 3), B allotransplantation tacrolimus monotherapy 7), C combined immunosuppression sirolimus 8), control D 6). Results: high rate complications occurred first group; 67% (12 18). group, reduced 12% (3 25). shortest survival found longest A. neither immunosuppressed groups (B + C) did any significant difference No complication healing group. conclusion can state safe kinds Vascular reconstruction vessels safer