3D imaging lipidometry in single cell by in-flow holographic tomography

• DOI: 10.29026/oea.2023.220048
• Publication Date: 2022-08-25T07:47:39Z
• Authors: Daniele Pirone; ; Daniele Sirico; Lisa Miccio; Vittorio Bianco; Martina Mugnano; Danila del Giudice; Gianandrea Pasqui...; Sabrina Valente; Silvia Lemma; Luisa Iommarini; Ivana Kurelac; Pasquale Memmolo; Pietro Ferraro
• Keywords: Characterization; Live cell imaging; Single-Cell Analysis

The most recent discoveries in the biochemical field are highlighting increasingly important role of lipid droplets (LDs) several regulatory mechanisms living cells. LDs dynamic organelles and therefore their complete characterization terms number, size, spatial positioning relative distribution cell volume can shed light on roles played by LDs. Until now, fluorescence microscopy transmission electron assessed as gold standard methods for identifying due to high sensitivity specificity. However, such generally only provide 2D assays partial measurements. Furthermore, both be destructive with low productivity, thus limiting analysis large numbers a sample. Here we demonstrate first time capability 3D visualization full LD high-throughput tomographic phase-contrast flow-cytometer, using ovarian cancer cells monocyte lines models. A strategy retrieving significant parameters correlations inside each is reported. information gathered this new method could allow more depth understanding lead how correlated cellular functions.