Large-field objective lens for multi-wavelength microscopy at mesoscale and submicron resolution

• DOI: 10.29026/oea.2024.230212
• Publication Date: 2024-06-11T08:44:47Z
• Authors: Xin Xu; ; Qin Luo; Jixiang Wang; Yahui Song; Hong Ye; Xin Zhang; Yi He; Minxuan Sun; Ruobing Zhang; Guohua Shi
• Keywords: 0301 basic medicine; confocal laser scanning microscopy; 0303 health sciences; high resolution; multi-wavelength; large field-of-view; mesoscopic objective lens; QC350-467; Optics. Light; wide-field microscopy

Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view, typically around 1 mm. This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample, such as different brain areas. In addition, conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo. Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm, a numerical aperture of 0.5, and a working wavelength range from 400 to 1000 nm. We achieved a resolution of 0.74 μm in fluorescent beads imaging. The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system, a confocal laser scanning system, and a two-photon imaging system. This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution.