Effects of Hair Follicle Stem Cells on Partial-Thickness Burn Wound Healing and Tensile Strength

• DOI: 10.29252/ibj.24.2.99
• Publication Date: 2019-12-30T10:21:38Z
• Authors: Azar Babakhani; ; Malihe Nobakht; ; Hamidreza Pazoki ...; ; Mostafa Dahmardehei; ; Paria Hashemi; ; Javad Mohajer Ansari; ; Parisa Ramhormozi; ; Abazar Yari; ; Fatemeh Heidari
• Keywords: CD31

The recent improvements in wound healing have led to new strategies regenerative medicine. Burn is an important issue skin regeneration and has multiple indications for stem cell therapy. Hair follicle cells (HFSCs) are a highly promising source of use, as these accessible, active pluripotent adult cells.HFSCs the rat whisker were isolated, cultured, labeled with DiI. Flow cytometry method was used detect special markers HFSCs. Deep partial-thickness burn created, HFSCs injected around bed. Wound closure recorded via digital photographs. inflicted rats sacrificed at 3, 7, or 14 days post subsequent histological tensiometry analysis.Our results indicated that positive Nestin CD34 markers, but negative Kr15. Morphological photographs revealed rate accelerated cell-treated group compared other groups. In addition, faster re-epithelialization collagen deposition observed. immunohistochemical analysis suggested CD31 expression vascular density enhanced group. Further, tissue tensile strength increased HFSCs-treated comparison control group.The present study demonstrates HFSC could accelerate well rats.