Effects of Hair Follicle Stem Cells on Partial-Thickness Burn Wound Healing and Tensile Strength

Male Pluripotent Stem Cells 0301 basic medicine Soft Tissue Injuries Full Length Cell- and Tissue-Based Therapy wound healing Antigens, CD34 Nestin 03 medical and health sciences Tensile Strength Animals Regeneration Rats, Wistar burn wound healing Skin Wound Healing R Rats 3. Good health Platelet Endothelial Cell Adhesion Molecule-1 tensile strength epithelization Medicine Collagen Burns Hair Follicle
DOI: 10.29252/ibj.24.2.99 Publication Date: 2019-12-30T10:21:38Z
ABSTRACT
The recent improvements in wound healing have led to new strategies in regenerative medicine. Burn wound healing is an important issue in skin regeneration and has multiple indications for stem cell therapy. Hair follicle stem cells (HFSCs) are a highly promising source of stem cells for healing use, as these cells are accessible, active and pluripotent adult stem cells.HFSCs of the rat whisker were isolated, cultured, and labeled with DiI. Flow cytometry method was used to detect special markers of HFSCs. Deep partial-thickness burn wound was created, and labeled HFSCs were injected around the wound bed. Wound closure was recorded via digital photographs. The inflicted rats were sacrificed at 3, 7, or 14 days post burn and used for subsequent histological and tensiometry analysis.Our results indicated that HFSCs were positive for Nestin and CD34 markers, but negative for Kr15. Morphological and histological photographs revealed that wound closure rate was accelerated in stem cell-treated group compared with other groups. In addition, faster re-epithelialization and collagen deposition were observed. The immunohistochemical analysis suggested that CD31 expression and vascular density enhanced in the stem cell-treated group. Further, tissue tensile strength increased in HFSCs-treated rats in comparison to the control group.The present study demonstrates that HFSC could accelerate burn wound healing as well as tensile strength in rats.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (0)
CITATIONS (32)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....