Production of reactive oxygen species by neutrophils and macrophages of F1 hybrid mice (C57Bl6xCBA) in response to stimulation with cucurbit(n)urils (n = 6, 7, 8)

• DOI: 10.29413/abs.2022-7.5-1.26
• Publication Date: 2022-12-14T11:18:15Z
• Authors: A. A. Aktanova; E. A. Pashkina; O. S. Boeva; N. A. Feofanova; V. A. Kozlov
• Keywords: Formazan; Nitroblue tetrazolium

Background. Due to their very small size, nanomaterials, in particular cucurbiturils, have unique physical and chemical properties that find application medicine. However, the toxicity of cucurbiturils is not fully understood; particular, we are interested immunological safety use. One mechanisms nanotoxicity formation reactive oxygen species (ROS) by macrophages neutrophils. Hyperproduction ROS can lead oxidative stress further damage cell DNA with loss physiological function development pathology. The aim. Evaluation effect cucurbit[n]urils (n = 6, 7, 8) on production mice Materials methods. F1 hybrid (CBAxC57Bl/6) aged 2 months 11) were used work. superoxide radical peritoneal mouse neutrophils was carried out spectrophotometric method for determining reduction p-nitroblue tetrazolium (NBT) formazan. Results. It shown CB[6] CB[7] at concentrations 0.5 0.3 mM do an inhibitory synthesis, but, contrary, significantly increase macrophages. In addition, increases level Conclusion. Cucurbiturils immunocompetent cells, depending concentration (0.3 higher).