PET of Glial Metabolism Using 2-18F-Fluoroacetate

• DOI: 10.2967/jnumed.108.057356
• Publication Date: 2009-05-15T00:56:24Z
• Authors: Jan Marik; Annie Ogasawara; Baby Martin-McNulty; Jed Ross; Judith E. Flores; Herman S. Gill; Jeff N. Tinianow; Alexander N. Vand...; Merry Nishimura; Franklin Peale; Cinthia Pastuskovas; Joan M. Greve; Nicholas van Bruggen; Simon P. Williams
• Keywords: Fluoroacetate

Imaging of the glial activation that occurs in response to central nervous system trauma and inflammation could become a powerful technique for assessment several neuropathologies. The selective uptake metabolism 2-(18)F-fluoroacetate ((18)F-FAC) glia may represent an attractive strategy imaging metabolism.We have evaluated use (18)F-FAC as specific PET tracer cell rodent models glioblastoma, stroke, ischemia-hypoxia.Enhanced was observed (6.98 +/- 0.43 percentage injected dose per gram [%ID/g]; tumor-to-normal ratio, 1.40) orthotopic U87 xenografts, compared with healthy brain tissue. lesion extent determined by correlated MRI (R(2) = 0.934, P 0.007). After transient middle cerebral artery occlusion rat brain, elevated (1.00 0.03 %ID/g; lesion-to-normal 1.90) depicted ischemic territory infarct volumes 2,3,5-triphenyltetrazolium chloride staining 0.692, 0.010) presence activated astrocytes detected anti-glial fibrillary acidic protein. Ischemia-hypoxia, induced permanent ligation common carotid hypoxia, resulted persistent elevation within 30 min induction hypoxia.Our data support further evaluation associated neuroinflammation.