In Vivo PET/CT in a Human Glioblastoma Chicken Chorioallantoic Membrane Model: A New Tool for Oncology and Radiotracer Development

• DOI: 10.2967/jnumed.112.117150
• Publication Date: 2013-08-23T04:09:17Z
• Authors: Geoff Warnock; Andrei Turtoi; Arnaud Blomme; Florian Bretin; Mohamed Ali Bahri; Christian Lemaire; Lionel Cyrille Li...; Alain E.J.J. Seret; André Luxen; Vincenzo Castronovo; Alain R.E.G. Plen...
• Keywords: Chorioallantoic membrane; U87; PET Imaging

For many years the laboratory mouse has been used as standard model for in vivo oncology research, particularly development of novel PET tracers, but growth tumors on chicken chorioallantoic membrane (CAM) provides a more rapid, low cost, and ethically sustainable alternative. first time, to our knowledge, we demonstrate feasibility CT imaging U87 glioblastoma tumor CAM, with aim applying this screening tracers.U87 cells were implanted CAM at day 11 after fertilization imaged 18. A small-animal cell was maintain incubation allow anesthesia using isoflurane. Radiotracers injected directly into exposed vasculature. Sodium (18)F-fluoride validate protocol, demonstrating that image-degrading motion can be removed anesthesia. Tumor glucose metabolism (18)F-FDG, protein synthesis 2-(18)F-fluoro-l-tyrosine. Anatomic images obtained by contrast-enhanced CT, facilitating clear delineation tumor, tracer uptake versus embryo, accurate volume measurements.PET successfully demonstrated model. Catheterization blood vessels facilitated dynamic (18)F-FDG ability study over time individual tumors, improved accuracy measurements.We describe application PET/CT model, optimization typical protocols. valuable could prove useful high-throughput radiotracers.