In Vivo Dynamic Metabolic Changes After Transplantation of Induced Pluripotent Stem Cells for Ischemic Injury

• DOI: 10.2967/jnumed.115.171124
• Publication Date: 2016-08-26T01:41:28Z
• Authors: Shuang Wu; Yuankai Zhu; Hao Liu; Ling Tang; Ruili Du; Yehua Shen; Jin Feng; Kai Zhang; Caiyun Xu; Shouhong Zhang; Yao Chen; Fahuan Song; Yunqi Zhu; Weizhong Gu; Ping Liang; Ignasi Carrió; Hong Zhang; Mei Tian
• Keywords: Male; 0301 basic medicine; metabolism myocardial ischemia; Induced Pluripotent Stem Cells; Myocardial Ischemia; Rats; 3. Good health; induced PSC-derived cardiomyocytes (iPSC-CMs); Rats, Sprague-Dawley; Electrocardiography; 03 medical and health sciences; Fluorodeoxyglucose F18; Positron-Emission Tomography; induced pluripotent stem cell (iPSC); Animals; positron emission tomography (PET)

This study aimed to investigate in vivo dynamic metabolic changes after transplantation of induced pluripotent stem cells (iPSCs) and iPSC-derived enriched cardiomyocytes (iPSC-CMs) a rat model ischemic injury.Serial 18F-FDG PET, echocardiographic, immunohistochemical, immunofluorescence studies were performed iPSCs iPSC-CMs compared with embryonic (ESCs), ESC-CMs, phosphate-buffered saline control group rats myocardial infarction.Increased glucose metabolism periinfarct areas improved function observed the cell groups group, serial immunohistochemical results exhibited survival migration during period.Serial PET echocardiographic imaging demonstrated recovery transplantation. could be potential approach evaluating spatiotemporal or for injury.