Tibial plateau levelling osteotomy: significance of matrix metalloproteinases in long-term monitoring of canine stifle stabilization after cranial cruciate ligament rupture.

• DOI: 10.30466/vrf.2023.2017099.4089
• Publication Date: 2025-01-01
• Authors: D. Ristanović; Jelena M. Živković; Milena Radaković; Kristina Spariosu; Ivan Vlahek; Marija Lipar; Petar Milosavljević; Mario Kreszinger

Cranial cruciate ligament rupture is one of the most common causes osteoarthritis in dogs. Surgical stabilization obligatory and tibial plateau levelling osteotomy (TPLO) commonly used surgical technique. Studies on long-term monitoring matrix metallo-proteinases (MMPs) acute-phase proteins are limited, especially those with parallel changes serum, synovial fluid (SF) urine. We aimed to describe 1) MMPs: gelatinases caseinases, 2) APPs: ceruloplasmin, haptoglobin paraoxonase-1, 3) correlation MMPs SF urine lameness dogs 2 6 months undergoing TPLO. From 17 diagnosed cranial rupture, sera, samples were collected preoperative after surgery. Relative activity (RA) was measured all using zymography. Acute-phase serum spectrophotometry agarose gel electrophoresis. not change at different sampling points. In SF, a marked decrease RA evident, however, only caseinases significantly reduced during recovery period. urine, positively correlated score decreasing trend time. Serum changed surgery, which consistent analyses. Decreased pointed degradation extracellular Synovial potential biomarkers predicting outcome following stifle stabilization.