Analgesic Efficacy of Subcutaneous–Oral Dosage of Tramadol after Surgery in C57BL/6J Mice

• DOI: 10.30802/aalas-jaalas-17-000118
• Publication Date: 2018-06-05T21:06:28Z
• Authors: Rocio Evangelista...; Alessandra Bergadano; Margarete Arras; Paulin D Jirkof
• Keywords: Male; Laparotomy; Pain, Postoperative; Injections, Subcutaneous; 610 Medicine & health; 04 agricultural and veterinary sciences; 3. Good health; Analgesics, Opioid; Mice, Inbred C57BL; 0403 veterinary science; 10021 Department of Trauma Surgery; Mice; Laboratory Animal Science; Animals; Pain Management; Animal Science and Zoology; Female; U10 Animal Welfare and 3Rs; 1103 Animal Science and Zoology; Analgesia; Tramadol; Pain Measurement

This study investigated the analgesic activity of tramadol in female C57BL/6J mice by using a single subcutaneous injection (25 mg/kg) combined with same dose given drinking water for 24 h. We then evaluated pharmacokinetics and its active metabolite O-demethyltramadol (M1). To evaluate pain efficacy, we performed clinical behavioral assessment, burrowing tests, analysis measured body weight, food intake that were untreated (control) or underwent analgesia only (T); anesthesia surgery (AS); anesthesia, surgery, (AS+T). The plasma concentration decreased rapidly whereas, more than 18 h, M1 level remained stable above minimal humans. Total over h was comparable among all groups. Although T consumed tramadol-treated sufficient amount frequency, AS AS+T animals showed frequency during first 4 after surgery. Compared control groups, composite scores latencies increased significantly both suggesting postsurgical pain. However, did not differ In conclusion, although naïve ingested drug levels appeared sufficiently high, markedly reduced immediately Consequently, even combination an initial injection, subsequent voluntary insufficient to reduce signs laparotomy.