Methyl Jasmonate and Nitric Oxide in Regulation of the Stomatal Apparatus of Arabidopsis thaliana

• DOI: 10.3103/s0095452718060129
• Publication Date: 2018-12-18T13:29:34Z
• Authors: T. O. Yastreb; Yu. E. Kolupaev; A. I. Kokorev; E. I. Horielova; A. P. Dmitriev
• Keywords: Sodium nitroprusside; Sodium nitrite; Methyl jasmonate

The effect of methyl jasmonate, donors and antagonists of nitric oxide (NO) on the state of stomata of Arabidopsis thaliana plants (Col-0) was studied. Treatment of the epidermis of leaves with a 50–400 μM solution of methyl jasmonate (MJ) caused a decrease in the size of the stomatal aperture and the percentage of open stomata. These effects were completely eliminated after preliminary treatment of epidermis cells with NO scavenger PTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) and partially after treatment with inhibitors of animal NO synthase (NG-nitro-L-arginine methyl ester, L-NAME) and nitrate reductase (sodium tungstate). NO donors (L-arginine and sodium nitrite) in concentrations of 0.5–2.0 mM also caused a decrease in the number of open stomata and the size of the stomatal aperture. The action of L‑arginine on the state of stomata was leveled by pretreatment of cells with L-NAME, and effects of sodium nitrite were eliminated by preaction of sodium tungstate. A conclusion about the possible participation of nitrogen oxide, forming both as the result of L-arginine oxidation and during nitrate/nitrite reduction, in the regulation of the stomatal apparatus and about its role in the realization of an MJ effect on state of stomata in Arabidopsis thaliana was made.