Increased anti-tumour activity by exosomes derived from doxorubicin-treated tumour cells via heat stress

• DOI: 10.3109/02656736.2015.1036384
• Publication Date: 2015-05-08T13:22:43Z
• Authors: Yunshan Yang; Yinghu Chen; Fanghui Zhang; Qiyu Zhao; Haijun Zhong
• Keywords: MTT assay

Purpose: Tumour-cell-derived exosomes (Exo) have been proposed as a new kind of drug carrier, and heat stress can promote release from tumour cells. This study investigated the impact on quantity doxorubicin in same number doxorubicin-treated MFC-7 cells their anti-tumour effects.Materials methods: Exosomes were isolated phosphate-buffered saline (Exo), (Exo-Dox) or combined with heat-stress-treated (Exo-Dox-HS) MCF-7 The content was determined by flow cytometry. effects individual types cell proliferation apoptosis well growth MTT assay, cytometry murine xenograft modelling.Results: We found that amount Exo-Dox-HS higher than Exo-Dox cells, contained levels Exo-Dox-HS, but not Exo 10 µg/mL doxorubicin, significantly inhibited triggered apoptosis, associated increased cleaved caspase-3 -8 morphological changes Treatment implanted breast tumours mice.Conclusions: Our indicated doxorubicin-containing enhanced effect findings may aid designing strategies for cancer therapy combination chemotherapy hyperthermia.