Fibroblast Growth Factor 23 and Klotho Are Present in the Growth Plate

• DOI: 10.3109/03008207.2012.753879
• Publication Date: 2012-12-03T13:25:47Z
• Authors: Adalbert Raimann; Diana Alexandra Ertl; Magdalena Helmreich; Susanne Sagmeister; Monika Egerbacher; Gabriele Haeusler
• Keywords: Klotho

Regulation of phosphate homeostasis is essential for mineralization and enchondral ossification. Fibroblast growth factor 23 (FGF23) its obligatory co-receptor Klotho (KL) play a key role in this process by influencing both renal reabsorption vitamin D metabolism. In disease, excessive action FGF23 leads to hypophosphatemic rickets, while deficiency causes tumoral calcinosis. Although osteocytes osteoblasts are widely seen as the primary source under physiological conditions, origin systemic remains controversial. study, we investigated expression KL porcine plate cartilage, adjacent tissues, parenchymal tissues.Tissue samples were obtained from 4- 6-week-old piglets. mRNA was quantified real-time PCR normalized 18S rRNA. Immunohistochemical staining performed FGF23, KL, collagen type X, FGF receptor 1. Growth chondrocyte subpopulations acquired collagenase digestion explants subsequent density gradient centrifugation.We could detect protein chondrocytes. mainly found hypertrophic resting Furthermore, significant genes observed bone, liver, spleen.These data challenge previous analyses, particular theories bone exclusive FGF23. Moreover, within tissues imply potential local differentiation tissue mineralization.