The protective effect of nesfatin-1 against renal ischemia–reperfusion injury in rats

• DOI: 10.3109/0886022x.2015.1015426
• Publication Date: 2015-02-24T13:02:46Z
• Authors: Guanjun Jiang; Min Wang; Lei Wang; Hui Chen; Zhiyuan Chen; Jia Guo; Xiaodong Weng; Xiuheng Liu
• Keywords: Renal Injury; Renal ischemia

The pathogenetic mechanisms underlying ischemia-reperfusion (I/R) injury involve oxidative stress, inflammation and apoptosis. Nesfatin-1, a novel peptide, has been reported to possess antioxidant, anti-inflammatory anti-apoptic properties. study was examine the potential protective effects of nesfatin-1 on renal I/R injury.I/R model induced by placing clamp across left artery for 45 min followed 24 h reperfusion, along with contralateral nephrectom. Twenty-four rats divided into three groups: sham-operated group, vehicle-treated nesfatin-1-treated I/R. Nesfatin-1 intraperitoneally injected 30 before ischemia. We harvested serum kidneys at after reperfusion. Renal function histological changes were assessed. Marker stress cells in kidney also evaluated.The animals significantly improved functional histologic lesions injury. malondialdehyde (MDA) level decreased, whereas superoxide dismutase (SOD) catalase (CAT) activities increased. Moreover, had markedly decrease apoptotic tubular cells, as well caspase-3 activity an increase bcl-2/Bax ratio.This is first evidence that treatment ameliorates acute suppressing cell Therefore, it promising therapeutic agent IR