Effects of crystalline glucocorticoid triamcinolone acetonide on cultered human supraspinatus tendon cells

• DOI: 10.3109/17453670902988360
• Publication Date: 2009-08-13T22:09:53Z
• Authors: Herbert Tempfer; Renate Gehwolf; Christine Lehner; Andrea Wagner; Maia Mtsariashvili; Hans-Christian Bauer; Herbert Resch; Mark Tauber
• Keywords: TIMP1

Rotator cuff tears are a common cause of shoulder pain and impairment. Subacromial glucocorticoid injections widely used for treatment epiphenomenons chronic impingement syndrome with the possible side effects tendon rupture impaired healing.Using qRT-PCR, western blot, immunoflourescence, measurement 3H-thymidine uptake we investigated crystalline triamcinolone acetonide (TAA) when added to culture medium isolated human rotator cells.After 2 weeks incubation, cells had lost their fibroblastic appearance parallel orientation, which is characteristic cellular degeneration in vivo. Moreover, expression secretion collagen I was strongly reduced, there decrease proliferation rate. Cell migration blocked rate matrix metalloproteinases MMP2, MMP8, MMP9, MMP13 but TIMP1 (a tissue inhibitor MMPs) upregulated, indicating reduction capacity repair. In addition, changes differentiation were observed: number adipocytes increased levels protein Sox9-a marker differentiating mature chondrocytes-were elevated treated cells.These results may indicate that use TAA one reason weaker mechanical properties high re-rupture after supraspinatus