Suitability of GFP-transformed isolates of the fungal root endophyte <i>Acremonium strictum</i> W. Gams for studies on induced <i>Fusarium</i>-wilt resistance in flax

• DOI: 10.3117/plantroot.1.46
• Publication Date: 2007-06-14T12:12:27Z
• Authors: Gisela Grunewaldt...; Nadine Riediger; Christof Dietrich
• Keywords: Acremonium; Endophyte; Wilt disease

Plant associated endophytic Acremonium species are capable of interacting with the host plant and altering its response towards pathogens pests. Specialized root-colonizing spp. can achieve biocontrol effects based on induced disease resistance in whole assays. However, these fungi hard to detect study root tissue by classical methods light microscopy or microbiology. To enable further progress investigations mechanisms defence plant, better visualization symbiont strictum was attempted use autofluorescent GFP-transformants. Subcultures three stable transformed A. strictum-isolates were used agar plate assays sterilized flax seedlings document colonization infection confocal laser scanning microscopy. In greenhouse tests fitness transformants proved comparison wild type for bioprotection against Fusarium-wilt. trials endophyte trait reduce incidence symptom severity verified all as well strictum. The suitability research scopes indicated.