Real-time measurement of tumour hypoxia using an implantable microfabricated oxygen sensor

• DOI: 10.31224/osf.io/fhqd7
• Publication Date: 2020-06-12T17:13:43Z
• Authors: Jamie R K Marland; Mark E Gray; Camelia Dunare; Ewen O Blair; Andreas Tsiamis; Paul Sullivan; Eva González-Fer...; Stephen N Greenhalgh; Rachael Gregson; R Eddie Clutton; Magdalena M Parys; Alex Dyson; Mervyn Singer; Ian H Kunkler; Mark A Potter; Srinjoy Mitra; Jonathan G Terry; Stewart Smith; Andrew R Mount; Ian Underwood; Anthony J Walton; David J Argyle; Alan F Murray
• Keywords: Oxygen sensor; Hypoxia

Hypoxia commonly occurs within tumours and is a major cause of radiotherapy resistance. Clinical outcomes could be improved by locating selectively increasing the dose delivered to hypoxic regions. Here we describe miniature implantable sensor for real-time monitoring tissue oxygenation that enable this novel treatment approach implemented. The uses solid-state electrochemical cell was microfabricated at wafer level on silicon substrate, includes an integrated reference electrode electrolyte membrane. It gave linear response oxygen concentration, unaffected sterilisation irradiation, but showed susceptibility biofouling. Oxygen selectivity also evaluated against various clinically relevant electroactive compounds. We investigated its robustness functionality under realistic clinical conditions using sheep model lung cancer. remained functional following CT-guided tumour implantation, sufficiently sensitive track acute changes in tissue.